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The Boom method (Boom nucleic acid extraction method) [1][2][3][4][5][6][7][8] is a solid phase extraction method for isolating nucleic acids (NA) [9] from biological samples. Silica beads are a key element to this method, which are capable of binding the nucleic acids in the presence of a chaotropic substance according to the chaotropic effect ...
Promega was the first company to provide kits for STR analysis of single loci. [10] Along with Applied Biosystems, Promega participated with the FBI and other crime labs in validating STR loci that would eventually be selected as the core loci for the Combined DNA Index System (CODIS), used for forensic DNA testing in North America. [11]
DNA extraction. The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other ...
Spin column-based nucleic acid purification. Silica in a spin column with water and with DNA sample in chaotropic buffer. Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions.
Plasmid miniprep. 0.8% agarose gel ethidium bromide -stained. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology. [1][2] Many methods have been developed to purify ...
Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional polymerase chain reaction methods that can be used to directly quantify and clonally amplify nucleic acids strands including DNA, cDNA, or RNA. The key difference between dPCR and qPCR lies in the method of measuring ...
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