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Semen analysis is a complex test that should be performed in andrology laboratories by experienced technicians with quality control and validation of test systems. A routine semen analysis should include: physical characteristics of semen (color, odor, pH, viscosity and liquefaction), volume, concentration, morphology and sperm motility and ...
Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA fragmentation. [1] First described by Evenson in 1980, the assay is a flow cytometric test that detects the vulnerability of sperm DNA to acid-induced denaturation DNA in situ. [2]
The postcoital test (PCT), also known as the Sims test, Hühner test ,Sims–Hühner test, or cervical mucous penetration assay is a test in the evaluation of infertility. The test examines interaction between sperm and mucus of the cervix .
Since the test is performed with fresh semen and the incubation requires only 10 minutes, it renders MAR test a quick and simple screening tool for ASA in human ejaculate. However, samples with very low sperm count (i.e. severe oligoastheno -, or even azoospermia ) cannot be evaluated using this method.
To perform the test, a drop of the reagent sodium alpha-napthyphosphate is added to the presumptive stain followed by a drop of fast blue B. A positive result of this test is a color change to dark purple. [4] [3] Confirmatory tests for semen include the christmas tree stain and the p30/PSA RSID kit.
A common route to diagnosis for hypospermia that may be requested for is a semen analysis. To obtain the most fresh specimen collection, a semen sample is obtained through the process of masturbation in the setting of a laboratory. An abstinence period of 3–5 days is recommended prior to collection of samples.