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The key criterion for the use of a housekeeping gene in this manner is that the chosen housekeeping gene is uniformly expressed with low variance under both control and experimental conditions. Validation of housekeeping genes should be performed before their use in gene expression experiments such as RT-PCR.
Normalization of Western blot data is an analytical step that is performed to compare the relative abundance of a specific protein across the lanes of a blot or gel under diverse experimental treatments, or across tissues or developmental stages.
The following represent genes that should probably not be used for reference purposes: GUSB, [5] RPLP0, [5] TFRC, [5] GAPDH, HSP90, and β-actin. Although they were once considered as "housekeeping genes," recent data suggests that they are not as reliable as once thought. [1] [2]
Western blot workflow. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
A housekeeping gene is a gene that is required to maintain basic cellular function and so is typically expressed in all cell types of an organism. Examples include actin , GAPDH and ubiquitin . Some housekeeping genes are transcribed at a relatively constant rate and these genes can be used as a reference point in experiments to measure the ...
Due to the sequence conservation in housekeeping genes, MLST sometimes lacks the discriminatory power to differentiate bacterial strains, which limits its use in epidemiological investigations. To improve the discriminatory power of MLST, a multi-virulence-locus sequence typing (MVLST) approach has been developed using Listeria monocytogenes ...
[[Category:Genome templates]] to the <includeonly> section at the bottom of that page. Otherwise, add <noinclude>[[Category:Genome templates]]</noinclude> to the end of the template code, making sure it starts on the same line as the code's last character.
The binding of TBP to these promoters is facilitated by housekeeping gene regulators. [ 8 ] [ 9 ] Interestingly, transcription initiates within a narrow region at around 30 bp downstream of TATA box on TATA-containing promoters, [ 10 ] while transcription start sites of TATA-less promoters are dispersed within a 200 bp region.