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The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). [1] Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid ...
It combines the functions of a thermal cycler and a fluorimeter, enabling the process of quantitative PCR. The first quantitative PCR machine was described in 1993, [2] and two commercial models became available in 1996. By 2009, eighteen different models were offered by seven different manufacturers. [3]
This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler. The primer design for all primers pairs has to be optimized so that all primer pairs can work at the same annealing temperature during PCR.
It is an environmental stress test used in evaluating product reliability as well as in manufacturing to catch early-term, latent defects by inducing failure through thermal fatigue. External links [ edit ]
Wilmington, Delaware, United States, Transparency Market Research, Inc., July 30, 2024 (GLOBE NEWSWIRE) -- The battery cyclers market (배터리 사이클러 시장) was projected to attain US$ 750.0 million in 2023. It is likely to garner a 4.9% CAGR from 2024 to 2034, and by 2034, the market is expected to attain US$ 1.3 billion.
Complex thermal cyclers were constructed to perform the Klenow-based amplifications, but never marketed. Simpler machines for Taq-based PCR were developed, and on November 19, 1987, a press release announces the commercial availability of the "PCR-1000 Thermal Cycler" and "AmpliTaq DNA Polymerase".