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Automatic analysis of urine test strips using automated urine test strip analysers is a well-established practice in modern-day urinalysis. They can measure calcium , blood, glucose, bilirubin, urobilinogen, ketones, leukocytes, creatinine , microalbumin , pH, ascorbic acid and protein.
Ketones occur in three forms in the body: beta-hydroxybutyrate (BHB), acetone and acetoacetate. Test strips use sodium nitroprusside to detect acetoacetate, and those with a glycine additive can detect acetone; however, none detect BHB. The reaction of ketones with sodium nitroprusside in an alkaline medium turns the test pad purple.
A wide variety of companies manufacture ketone screening strips. A strip consists of a thin piece of plastic film slightly larger than a matchstick , with a reagent pad on one end that is either dipped into a urine sample or passed through the stream while the user is voiding.
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Test for ketonuria using Bayer Ketostix reagent strips. Urine testing is the most common method of testing for ketones. Urine test strips utilize a nitroprusside reaction with acetoacetate to give a semi-quantitative measure based on color change of the strip. Although beta-hydroxybutyrate is the predominant circulating ketone, urine test ...
This is performed using ketone test strips containing nitroprusside, which change colour from buff-pink to maroon in the presence of acetoacetate (one of the three ketone bodies). [47] A short-lived increase in seizure frequency may occur during illness or if ketone levels fluctuate.