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In organic chemistry, Fehling's solution is a chemical reagent used to differentiate between water-soluble carbohydrate and ketone (>C=O) functional groups, and as a test for reducing sugars and non-reducing sugars, supplementary to the Tollens' reagent test. The test was developed by German chemist Hermann von Fehling in 1849. [1]
In analytical chemistry, a reagent is a compound or mixture used to detect the presence or absence of another substance, e.g. by a color change, or to measure the concentration of a substance, e.g. by colorimetry. Examples include Fehling's reagent, Millon's reagent, and Tollens' reagent.
The structure of the fuchsin dye. The Schiff test is an early organic chemistry named reaction developed by Hugo Schiff, [1] and is a relatively general chemical test for detection of many organic aldehydes that has also found use in the staining of biological tissues. [2]
The aqueous solution in the classical reaction contains glucose, sodium hydroxide and methylene blue. [14] In the first step an acyloin of glucose is formed. The next step is a redox reaction of the acyloin with methylene blue in which the glucose is oxidized to diketone in alkaline solution [6] and methylene blue is reduced to colorless leucomethylene blue.
Benedict's reagent (often called Benedict's qualitative solution or Benedict's solution) is a chemical reagent and complex mixture of sodium carbonate, sodium citrate, and copper(II) sulfate pentahydrate. [1] It is often used in place of Fehling's solution to detect the presence of reducing sugars and other reducing substances. [2]
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The colour change of halochromic substances occur when the chemical binds to existing hydrogen and hydroxide ions in solution. [1] Such bonds result in changes in the conjugated systems of the molecule, or the range of electron flow. This alters the wavelength of light absorbed, which in turn results in a visible change of colour.