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DNA–DNA hybridization (DDH) is used as a primary method to distinguish bacterial species as it is difficult to visually classify them accurately. [7] This technique is not widely used on larger organisms where differences in species are easier to identify.
This method, which is commonly used with Mueller–Hinton agar, is used by evenly seeding bacteria over a petri dish and applying an antibiotic treated disk to the top of the agar. By observing the ring formed around the disk formed due to the lack of bacterial growth, the zone of inhibition can be found, which is used to find the ...
Microbial phylogenetics is the study of the manner in which various groups of microorganisms are genetically related. This helps to trace their evolution. [1] [2] To study these relationships biologists rely on comparative genomics, as physiology and comparative anatomy are not possible methods. [3]
Bacterial taxonomy is the classification of strains within the domain Bacteria into hierarchies of similarity. This classification is similar to that of plants , mammals , and other taxonomies. However, biologists specializing in different areas have developed differing taxonomic conventions over time.
Microbial genetics studies microorganisms for different purposes. The microorganisms that are observed are bacteria and archaea. Some fungi and protozoa are also subjects used to study in this field. The studies of microorganisms involve studies of genotype and expression system. Genotypes are the inherited compositions of an organism.
Genotyping is the process of determining differences in the genetic make-up of an individual by examining the individual's DNA sequence using biological assays and comparing it to another individual's sequence or a reference sequence.
The analytical profile index, or API, is a classification system for bacteria based on biochemical tests. The system was developed to accelerate the speed of identifying clinically relevant bacteria. It can only be used to identify known species from an index. [1] The data obtained are phenotypic traits.
Bergey's Manual Trust was established in 1936 to sustain the publication of Bergey's Manual of Determinative Bacteriology and supplementary reference works. The Trust also recognizes individuals who have made outstanding contributions to bacterial taxonomy by presentation of the Bergey Award and Bergey Medal, jointly supported by funds from the Trust and from Springer, the publishers of the ...