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The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours. The presence of some coliforms indicate fecal contamination.
Enterobacteriaceae is a large family of Gram-negative bacteria. It includes over 30 genera and more than 100 species. It includes over 30 genera and more than 100 species. Its classification above the level of family is still a subject of debate, but one classification places it in the order Enterobacterales of the class Gammaproteobacteria in ...
Sulphide Indole Motility (SIM) medium is a bacterial growth medium which tests for the ability to reduce sulfates, the ability to produce indoles, and motility. [1] This combination of challenges in one mixture is convenient and commercially available in stab tubes.
Before starting a test, one must confirm the cultured bacteria are Enterobacteriaceae, this is done by a quick oxidase test for cytochrome coxidase. Enterobacteriaceae are typically oxidase negative, meaning they either do not use oxygen as an electron acceptor in the electron transport chain , or they use a different cytochrome enzyme for ...
Simmons' citrate agar (not planted yet) Simmons' citrate agar is used for differentiating gram-negative bacteria on the basis of citrate utilization, [1] especially for distinguishing Gammaproteobacteria of the family Enterobacteriaceae or even between species of the same genus. [2]
Klebsiella aerogenes, [2] previously known as Enterobacter aerogenes, is a Gram-negative, oxidase-negative, catalase-positive, citrate-positive, indole-negative, rod-shaped bacterium. [3]
Klebsiella oxytoca on agar plate. Klebsiella oxytoca is a Gram-negative, rod-shaped bacterium that is closely related to K. pneumoniae, from which it is distinguished by being indole-positive; it also has slightly different growth characteristics in that it is able to grow on melezitose, but not 3-hydroxybutyrate.
Edwardsiella tarda is a member of the family Hafniaceae. [1] [2] The bacterium is a facultatively anaerobic, small, motile, gram negative, straight rod with flagella.[1] [2] Infection causes Edwardsiella septicemia (also known as ES, edwardsiellosis, emphysematous putrefactive disease of catfish, fish gangrene, and red disease) in channel catfish, eels, and flounder.