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2. Complementary DNA - Wikipedia

   en.wikipedia.org/wiki/Complementary_DNA

   [12] cDNA is commonly generated from mRNA for gene expression analyses such as RT-qPCR and RNA-seq. [13] mRNA is selectively reverse transcribed using oligo-dT primers that are the reverse complement of the poly-adenylated tail on the 3' end of all mRNA. The oligo-dT primer anneals to the poly-adenylated tail of the mRNA to serve as a binding ...

3. RNA-Seq - Wikipedia

   en.wikipedia.org/wiki/RNA-Seq

   The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput , short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed.

4. Single-cell sequencing - Wikipedia

   en.wikipedia.org/wiki/Single-cell_sequencing

   Overall, in a first stage individual cells are captured separately and lysed, then reverse transcription (RT) of mRNA is performed and cDNA library is obtained. To select mRNA, the RT is performed with a single-stranded sequence of deoxythymine (oligo dT) primer which bind specifically the poly(A) tail of mRNA molecules. Subsequently, the ...

5. Transcriptomics technologies - Wikipedia

   en.wikipedia.org/wiki/Transcriptomics_technologies

   The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In microarrays, the ds-cDNA is fragmented and fluorescently labelled (orange). The labelled fragments bind to an ordered array of complementary oligonucleotides, and measurement of fluorescent intensity across the array ...

6. DNA microarray - Wikipedia

   en.wikipedia.org/wiki/DNA_microarray

   The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In microarrays, the ds-cDNA is fragmented and fluorescently labelled (orange). The labelled fragments bind to an ordered array of complementary oligonucleotides, and measurement of fluorescent intensity across the array ...

7. cDNA library - Wikipedia

   en.wikipedia.org/wiki/CDNA_library

   A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism.

8. CITE-Seq - Wikipedia

   en.wikipedia.org/wiki/CITE-Seq

   In the next step, based on the developer's guidelines, cDNA is PCR-amplified and ADT cDNA and mRNA cDNA are separated based on size (generally, ADT-derived cDNAs are < 180bp and mRNA-derived cDNAs are > 300bp). [7] Each of the separated cDNA molecules is independently amplified and purified to prepare sequencing libraries.

9. Single-cell transcriptomics - Wikipedia

   en.wikipedia.org/wiki/Single-cell_transcriptomics

   Detecting differences in gene expression level between two populations is used both single-cell and bulk transcriptomic data. Specialised methods have been designed for single-cell data that considers single cell features such as technical dropouts and shape of the distribution e.g. Bimodal vs. unimodal. [23]