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Dried blood spot testing (DBS) is a form of biosampling where blood samples are blotted and dried on filter paper. The dried samples can easily be shipped to an analytical laboratory and analysed using various methods such as DNA amplification or high-performance liquid chromatography .
False positives and false negatives can sometimes occur when the screening tests are performed before 48 hours. [6] When the immunoassay method is utilized as a screening method for quantifying 17α-hydroxyprogesterone (17OHP) in dried blood spots, it exhibits a
With this testing methodology, newborn screening required one test to detect one condition. As mass spectrometry became more widely available, the technology allowed rapid determination of a number of acylcarnitines and amino acids from a single dried blood spot. This increased the number of conditions that could be detected by newborn screening.
whole blood: venipuncture: requires phlebotomist to collect Dried blood spot: gives high quality DNA and RNA Fingerstick: stores easily for years at room temperature organ tissue gives high quality DNA, RNA, Mitochondrial DNA, and source of disease Biopsy: many uses shared with blood; also suitable for proteomic analysis; may be difficult to ...
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