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New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. [2] It also provides products and services supporting genome editing, synthetic biology and next-generation sequencing. [3]
A restriction map is a map of known restriction sites within a sequence of DNA.Restriction mapping requires the use of restriction enzymes.In molecular biology, restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA.
A pUC19 cloning vector showing the multiple cloning site sequence with restriction enzyme sites. A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites - a standard feature of engineered plasmids. [1]
HpaII (IntEnz: EC 3.1.21.4) is a restriction enzyme obtained from the microorganism Haemophilus parainfluenzae.It is a DNA restriction enzyme, therefore it has the ability to cut the DNA from certain region as demonstrated below. [1]