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Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
Restriction digest is most commonly used as part of the process of the molecular cloning of DNA fragment into a vector (such as a cloning vector or an expression vector).The vector typically contains a multiple cloning site where many restriction site may be found, and a foreign piece of DNA may be inserted into the vector by first cutting the restriction sites in the vector as well the DNA ...
Genes and other genetic information from a wide range of organisms can be added to a plasmid and inserted into bacteria for storage and modification. Bacteria are cheap, easy to grow, clonal , multiply quickly, are relatively easy to transform, and can be stored at -80 °C almost indefinitely.
Plasmid-mediated resistance is the transfer of antibiotic resistance genes which are carried on plasmids. [1] Plasmids possess mechanisms that ensure their independent replication as well as those that regulate their replication number and guarantee stable inheritance during cell division.
From January 2008 to December 2012, if you bought shares in companies when Kenneth B. Woodrow joined the board, and sold them when he left, you would have a -19.4 percent return on your investment, compared to a -2.8 percent return from the S&P 500.
Seated alongside team owner Steve Cohen, president of baseball operations David Stearns and agent Scott Boras, Soto said he noticed what the Mets were doing from "the other side" of town.
Christopher Sepulvado, the 81-year-old man who was facing execution next month for the 1992 murder of his stepson, died overnight at Louisiana State Penitentiary in Angola, according to his attorney.
Claire Schwartz, who gathers photos blown and burned from the Eaton fire, is given a photo found by Nila Sinnatamay. Schwartz recognized the people in the photo and will return it to the owner.