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A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. [1]
3D cell culturing by Magnetic Levitation Method (MLM) is the culturing of 3D cell tissue by inducing cells treated with magnetic nanoparticle assemblies in spatially varying magnetic fields, using neodymium magnetic drivers and promoting cell-to-cell interactions by levitating the cells up to the air/liquid interface of a standard petri dish.
Currently, there is an increase in use of 3D cell cultures in research areas including drug discovery, cancer biology, regenerative medicine, nanomaterials assessment and basic life science research. [65] [66] [67] 3D cell cultures can be grown using a scaffold or matrix, or in a scaffold-free manner. Scaffold based cultures utilize an ...
Organ-on-a-chip. An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture, integrated circuit (chip) that simulates the activities, mechanics and physiological response of an entire organ or an organ system. [1][2] It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS.
A Hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which are small, semi-permeable capillary membranes arranged in parallel array with a typical molecular weight cut-off (MWCO) range of 10-30 kDa. These hollow fiber membranes are often bundled and housed within tubular polycarbonate shells to create ...
Left: multicellular hollow spheres of cells, termed acini, were grown in 3D culture. Right: after 2 days of treatment with HGF cells have formed multiple branches. In the last 20 years, understanding of MDCK cell biology in 3D culture has been advanced most notably by the laboratory of Keith Mostov. This group has focused on the regulation of ...