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Historically a cesium chloride (CsCl) solution was often used, but more commonly used density gradients are sucrose or Percoll.This application requires a solution with high density and yet relatively low viscosity, and CsCl suits it because of its high solubility in water, high density owing to the large mass of Cs, as well as low viscosity and high stability of CsCl solutions.
Centrifugal force separates components not only on the basis of density, but also of particle size and shape. In contrast, a more specialized equilibrium density-gradient centrifugation produces a separation profile dependent on particle-density alone, and therefore is suitable for more fine-grained separations.
Density gradient centrifugation is known to be one of the most efficient methods for separating suspended particles, and is used both as a separation technique and as a method for measuring the density of particles or molecules in a mixture. [17]
Isopycnic centrifugation refers to a method wherein a density gradient is either pre-formed or forms during high speed centrifugation. After this gradient is formed particles move within the gradient to the position having a density matching their own (this is in fact an incorrect description of the exact physical process but does describe the ...
Sedimentation equilibrium experiments reports the molar mass of analytes and their chemical equilibrium constants. [3] The rotor speed is adjusted such that a steady-state concentration profile c(r) of the sample in the cell is formed, where sedimentation and diffusion cancel out each other.
In order to test hypotheses for how DNA replicates, Meselson and Stahl, together with Jerome Vinograd, invented a method that separates macromolecules according to their buoyant density. [6] The method, equilibrium density gradient centrifugation, was sufficiently sensitive that Meselson and Stahl were able to separate DNA containing the heavy ...
When DNA is extracted from these cells and made to undergo buoyant density centrifugation on a salt density gradient, the DNA separates out at the point at which its density equals that of the salt solution. The DNA of the cells grown in 15 N medium had a higher density than cells grown in normal 14 N medium.
Concentration of Plasmodium falciparum-infected erythrocytes by discontinuous density gradient centrifugation in Percoll [1] Percoll is a reagent consisting of colloidal silica particles used in cell biology and other laboratory settings. It was first formulated by Pertoft and colleagues, [2] and commercialized by Pharmacia Fine Chemicals. [3]