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In biotechnology applications, T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two (different) phage promoters (e.g., T7 and T3, or T7 and SP6) in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with the different polymerases. The enzyme is stimulated ...
Soon, the lab was able to clone the T7 RNA polymerase and use it, along with the powerful T7 promoter, to transcribe copious amounts of almost any gene. [4] The development of the T7 expression system has been considered the most successful biotechnology developed at the Brookhaven National Laboratory, being licensed by over 900 companies which ...
The T7 promoter sequence is used extensively in molecular biology due to its extremely high affinity for T7 RNA polymerase and thus high level of expression. [3] [2] T7 has been used as a model in synthetic biology. Chan et al. (2005) "refactored" the genome of T7, replacing approximately 12 kbp of its genome with engineered DNA. [15]
Abortive cycling by T7 RNA polymerase. Abortive initiation, also known as abortive transcription, is an early process of genetic transcription in which RNA polymerase binds to a DNA promoter and enters into cycles of synthesis of short mRNA transcripts which are released before the transcription complex leaves the promoter.
T7 RNA polymerase producing a mRNA (green) from a DNA template. The protein is shown as a purple ribbon (Orthopoxviruses and some other nucleocytoplasmic large DNA viruses synthesize RNA using a virally encoded multi-subunit RNAP. They are most similar to eukaryotic RNAPs, with some subunits minified or removed. [45]
The Pribnow box has a function similar to the TATA box that occurs in promoters in eukaryotes and archaea: it is recognized and bound by a subunit of RNA polymerase during initiation of transcription. [3] This region of the DNA is also the first place where base pairs separate during prokaryotic transcription to allow access to the template strand.
Termination efficiency of T7 RNA polymerase is around 74%, which creates issues when T7 RNA polymerase is used to produce recombinant proteins. [3] In this process, the target gene is inserted into a plasmid and is regulated by the T7 promoter ; T7 RNA polymerase is used to transcribe the target gene. [ 3 ]
216151 Ensembl ENSG00000099821 ENSMUSG00000020329 UniProt O00411 Q8BKF1 RefSeq (mRNA) NM_005035 NM_172551 RefSeq (protein) NP_005026 NP_766139 NP_001394721 NP_001394722 NP_001394723 NP_001394724 NP_001394725 NP_001394726 NP_001394727 NP_001394728 Location (UCSC) Chr 19: 0.62 – 0.63 Mb Chr 10: 79.57 – 79.58 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse DNA-directed RNA ...