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  2. File:Workflow of isotope labeling experiment.pdf - Wikipedia

    en.wikipedia.org/wiki/File:Workflow_of_isotope...

    English: A labeled glucose tracer is introduced into the medium. After cells consume the labeled feed, they are lysed to extract metabolites. Intracellular metabolites are analyzed using a spectrometer instrument to ultimately obtain a spectrum of mass isotopomer and metabolite concentration data.

  3. Fluid mosaic model - Wikipedia

    en.wikipedia.org/wiki/Fluid_mosaic_model

    Fluid mosaic model of a cell membrane. The fluid mosaic model explains various characteristics regarding the structure of functional cell membranes.According to this biological model, there is a lipid bilayer (two molecules thick layer consisting primarily of amphipathic phospholipids) in which protein molecules are embedded.

  4. Stable isotope labeling by amino acids in cell culture

    en.wikipedia.org/wiki/Stable_isotope_labeling_by...

    Stable isotope labeling by/with amino acids in cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling.

  5. Isotopic labeling - Wikipedia

    en.wikipedia.org/wiki/Isotopic_labeling

    An isotopic label is fed to the cell, then the cell is allowed to grow utilizing the labeled feed. For stationary metabolic flux analysis the cell must reach a steady state (the isotopes entering and leaving the cell remain constant with time) or a quasi-steady state (steady state is reached for a given period of time). [7]

  6. Proximity labeling - Wikipedia

    en.wikipedia.org/wiki/Proximity_labeling

    Proximity labeling relies on a labeling enzyme that can biotinylate nearby biomolecules promiscuously. Biotin labeling can be achieved through several different methods, depending on the species of labeling enzyme. BioID, also known as BirA*, is a mutant E. coli biotin ligase that catalyzes the activation of biotin by ATP. The activated biotin ...

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  8. Immunolabeling - Wikipedia

    en.wikipedia.org/wiki/Immunolabeling

    Immunolabeling is a biochemical process that enables the detection and localization of an antigen to a particular site within a cell, tissue, or organ. Antigens are organic molecules, usually proteins , capable of binding to an antibody .

  9. Spreadsheet - Wikipedia

    en.wikipedia.org/wiki/Spreadsheet

    A worksheet is simply a subset of the spreadsheet divided for the sake of clarity. Functionally, the spreadsheet operates as a whole and all cells operate as global variables within the spreadsheet (each variable having 'read' access only except its containing cell). A cell may contain a value or a formula, or it may