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Protoplast (from Ancient Greek πρωτόπλαστος (prōtóplastos) 'first-formed'), is a biological term coined by Hanstein in 1880 to refer to the entire cell, excluding the cell wall. [ 1 ] [ 2 ] Protoplasts can be generated by stripping the cell wall from plant , [ 3 ] bacterial , [ 4 ] [ 5 ] or fungal cells [ 5 ] [ 6 ] by mechanical ...
If the bacterial cell wall is entirely removed, it is called a protoplast while if it's partially removed, it is called a spheroplast. Beta-lactam antibiotics such as penicillin inhibit the formation of peptidoglycan cross-links in the bacterial cell wall.
A spheroplast (or sphaeroplast in British usage) is a microbial cell from which the cell wall has been almost completely removed, as by the action of penicillin or lysozyme. According to some definitions, the term is used to describe Gram-negative bacteria. [3] [4] According to other definitions, the term also encompasses yeasts.
L-form bacteria were first isolated in 1935 by Emmy Klieneberger-Nobel, who named them "L-forms" after the Lister Institute in London where she was working. [2]She first interpreted these growth forms as symbionts related to pleuropneumonia-like organisms (PPLOs, later commonly called mycoplasmas). [3]
Bacteria display a large diversity of cell morphologies and arrangements.. Bacterial cellular morphologies are the shapes that are characteristic of various types of bacteria and often key to their identification.
Bacterial morphological plasticity refers to changes in the shape and size that bacterial cells undergo when they encounter stressful environments. Although bacteria have evolved complex molecular strategies to maintain their shape, many are able to alter their shape as a survival strategy in response to protist predators, antibiotics, the immune response, and other threats.
It is generally stated that fully turgid cells have a turgor pressure that is equal to that of the cell and that flaccid cells have a value at or near zero. Other cellular mechanisms to be taken into consideration include the protoplast, solutes within the protoplast (solute potential), transpiration rates of the cell and the tension of cell ...
Fused protoplast (left) with chloroplasts (from a leaf cell) and coloured vacuole (from a petal) Somatic fusion, also called protoplast fusion, is a type of genetic modification in plants by which two distinct species of plants are fused together to form a new hybrid plant with the characteristics of both, a somatic hybrid. [1]