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DNA nanoball sequencing is a type of high throughput sequencing technology used to determine the entire genomic sequence of an organism. The company Complete Genomics uses this technology to sequence samples submitted by independent researchers.
The ideal size of DNA fragments for the sequencing library depends on the sequencing platform that will be used. [4] [16] DNA can first be sheared to fragments around 300–500 bp long using sonication. [4] [16] [17] Fragments of this size are suitable for high-throughput sequencing.
Massive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing.
The later development by Leroy Hood and coworkers [7] [8] of fluorescently labeled ddNTPs and primers set the stage for automated, high-throughput DNA sequencing. Sequence ladder by radioactive sequencing compared to fluorescent peaks. Chain-termination methods have greatly simplified DNA sequencing.
The second step is to sequence the exonic DNA using any high-throughput DNA sequencing technology. [2] The goal of this approach is to identify genetic variants that alter protein sequences, and to do this at a much lower cost than whole-genome sequencing.
The Human Genome Project spurred the development of cheaper, high throughput and more accurate platforms known as Next Generation Sequencers (NGS) to sequence the human genome. These include the 454, SOLiD and Illumina DNA sequencing platforms. Next generation sequencing machines have increased the rate of DNA sequencing substantially, as ...
Hi-C uses high-throughput sequencing to find the nucleotide sequence of fragments [2] [22] and uses paired end sequencing, which retrieves a short sequence from each end of each ligated fragment. As such, for a given ligated fragment, the two sequences obtained should represent two different restriction fragments that were ligated together in ...
Since the inception of high‐throughput sequencing , [19] the use of metabarcoding as a biodiversity detection tool has drawn immense interest. [12] [20] However, there has yet to be clarity regarding what source material is used to conduct metabarcoding analyses (e.g., environmental DNA versus community DNA). Without clarity between these two ...