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A buccal swab, also known as buccal smear, is a way to collect DNA from the cells on the inside of a person's cheek.Buccal swabs are a relatively non-invasive way to collect DNA samples for testing.
DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components.
Chelex resin is often used for DNA extraction in preparation for polymerase chain reaction by binding to cations including Mg 2+, which is an essential cofactor for DNases. Chelex protects the sample from DNases that might remain active after the boiling and could subsequently degrade the DNA, rendering it unsuitable for PCR.
The video shows the process of extracting genotypes from a human spit sample using a DNA microarray, which is the most common method used in genetic genealogy. A genealogical DNA test is performed on a DNA sample obtained by cheek-scraping (also known as a buccal swab), spit-cups, mouthwash, or chewing gum.
The most common methods of DNA extraction include organic extraction (also called phenol–chloroform extraction), [17] Chelex extraction, and solid-phase extraction. Differential extraction is a modified version of extraction in which DNA from two different types of cells can be separated from each other before being purified from the solution ...
Date/Time Thumbnail Dimensions User Comment; current: 12:22, 6 April 2014: 3 min 52 s, 480 × 360 (7.38 MB): Jacopo Werther == {{int:filedesc}} == {{Information |Description = Step-by-step video and audio instructions on how to prepare a wet mount specimen of eukaryotic animal cells; specifically Human epithelial cells from the inside of the cheek.