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Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet.Thereafter, the tissue is grown in sterile containers, such as Petri dishes or flasks in a growth room with controlled temperature and light intensity.
Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.
Other identified plant growth regulators include: Plant peptide hormones – encompasses all small secreted peptides that are involved in cell-to-cell signaling. These small peptide hormones play crucial roles in plant growth and development, including defense mechanisms, the control of cell division and expansion, and pollen self ...
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is the most popular plant growth medium used in the laboratories worldwide for cultivation of plant cell culture on agar. MS0 was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS ...
Certain compounds excreted by plant tissue cultures and found in culture media have been shown necessary to coordinate cell division and morphological changes. [9] These compounds have been identified by Chung et al. [ 10 ] as various polysaccharides , amino acids , growth regulators , vitamins , low molecular weight compounds and polypeptides.
Kinetin is often used in plant tissue culture to induce callus formation (in conjunction with auxin) and regenerate shoot tissues from callus (with lower auxin concentration). For a long time, it was believed that kinetin was an artifact produced from the deoxyadenosine residues in DNA , which degraded when standing for long periods or when ...
They are known to regulate axillary bud growth and apical dominance. According to the "direct inhibition hypothesis", these effects result from the ratio of cytokinin to auxin. [citation needed] This theory states that auxin from apical buds travels down shoots to inhibit axillary bud growth. This promotes shoot growth, and restricts lateral ...
In plant tissue culture IBA and other auxins are used to initiate root formation in vitro in a procedure called micropropagation.Micropropagation of plants is the process of using small samples of plants called explants and causing them to undergo growth of differentiated or undifferentiated cells.