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Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis , [ 2 ] Francisella and Nocardia spp .
Schädler agar is a nutrient-rich growth medium primarily used in microbiology for the cultivation of anaerobic bacteria. It was developed to support the growth of a wide variety of anaerobic organisms, providing a conducive environment for both fastidious and non-fastidious anaerobes . [ 1 ]
Nutrient agar is a general-purpose solid medium supporting growth of a wide range of non-fastidious organisms. It typically contains ( mass/volume ): [ 1 ] 0.5% peptone – this provides organic nitrogen
The total number of living aerobic bacteria can be determined using a plate count agar which is a substrate for bacteria to grow on. The medium contains casein which provides nitrogen, carbon, amino acids, vitamins and minerals to aid in the growth of the organism. Yeast extract is the source for vitamins, particularly of B-group. Glucose is ...
11.0 g Agar; Preparation: 1. Heat with frequent agitation and boil for 1 minute to completely dissolve. 2. Autoclave at 121 °C for 15 minutes. Cool to 50 °C. 3. Add 50 ml filter sterilized 10% lactose solution and mix well (the lactose can be exchanged to other carbohydrates e.g. glucose, resulting in GM17 medium)
The first culture media was liquid media, designed by Louis Pasteur in 1860. [2] This was used in the laboratory until Robert Koch's development of solid media in 1881. [ 3 ] Koch's method of using a flat plate for his solid media was replaced by Julius Richard Petri's round box in 1887. [ 2 ]