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  2. Real-time polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Real-time_polymerase_chain...

    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR.

  3. MIQE - Wikipedia

    en.wikipedia.org/wiki/MIQE

    The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments and data, as devised by Bustin et al. in 2009. [1]

  4. Melting curve analysis - Wikipedia

    en.wikipedia.org/wiki/Melting_curve_analysis

    Melting curve analysis is an ... Applied Biosystems 7500 Fast System and the 7900HT Fast Real-Time PCR System, Idaho Technology's LightScanner (the first plate-based ...

  5. High-resolution melting analysis - Wikipedia

    en.wikipedia.org/wiki/High-resolution_melting...

    The use of HRM reduces the time required for analysis and the risk of contamination. HRM is a more cost-effective solution and the high resolution element not only allows the determination of homo and heterozygosity , it also resolves information about the type of homo and heterozygosity, with different gene variants giving rise to differing ...

  6. Reverse transcription polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Reverse_transcription...

    It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article, RT-PCR will denote Reverse ...

  7. Relative fluorescence units - Wikipedia

    en.wikipedia.org/wiki/Relative_fluorescence_units

    The RFU measurements are used, for DNA profiling, in a real-time polymerase chain reaction (PCR). Two common methods for detection of products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labeled with a fluorescent reporter which permits detection only after ...