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Equipment used in aseptic processing of food and beverages must be sterilized before processing and remain sterile during processing. [1] When designing aseptic processing equipment there are six basic requirements to consider: the equipment must have the capability of being cleaned thoroughly, it must be able to be sterilized with steam, chemicals, or high-temperature water, sterilization ...
Basic aseptic procedures includes hand washing, donning protective gloves, masks and gowns, and sterilizing equipment and linens. [12] Medical aseptic techniques also includes curbing the spread of infectious diseases through quarantine, specifically isolation procedures based on the mode of disease transmission. [12]
Using an aseptic technique, bacteria are placed on a slide and heat fixed. The slide is then suspended over a water bath with some sort of porus paper over it, so that the slide is steamed. Malachite green is applied to the slide, which can penetrate the tough walls of the endospores, staining them green.
Aseptic techniques are used to maintain microbiological cultures and to prevent contamination of the growth medium. There are many different types of methods used to streak a plate. Picking a technique is a matter of individual preference and can also depend on how large the number of microbes the sample contains.
Pressure sterilization is the prevailing method used for medical sterilization of heat-resistant tools, and for sterilization of materials for microbiology and other fields calling for aseptic technique. [citation needed] In cases when items need to be sterilized for immediate use, flash sterilization may be employed. [3]
Microorganisms growing on an agar plate. Sterilization (British English: sterilisation) refers to any process that removes, kills, or deactivates all forms of life (particularly microorganisms such as fungi, bacteria, spores, and unicellular eukaryotic organisms) and other biological agents (such as prions or viruses) present in fluid or on a specific surface or object. [1]
Aseptic technique is a key component of all invasive medical procedures. Similar control measures are also recommended in any healthcare setting to prevent the spread of infection generally. Similar control measures are also recommended in any healthcare setting to prevent the spread of infection generally.
Using aseptic technique, broth culture of a specific organism is collected with a sterile swab. In the case of Gram negative bacteria, excess liquid is removed from the swab by gently pressing or rotating it against the inside of the tube. The swab is then streaked across a Mueller–Hinton agar plate to form a bacterial lawn.