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  2. Ultraviolet–visible spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Ultraviolet–visible...

    A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.

  3. Job plot - Wikipedia

    en.wikipedia.org/wiki/Job_plot

    In the case of UV-visible spectroscopy, for example, this means that the system must conform to the Beer-Lambert law. In addition, the total concentration of the two binding partners, the pH and ionic strength of the solution must all be maintained at fixed values throughout the experiment.

  4. DU spectrophotometer - Wikipedia

    en.wikipedia.org/wiki/DU_spectrophotometer

    [2]: 151 [34] [35] All modern UV–Vis spectrophotometer are built on the same basic principles as the DU spectrophotometer. [29] Diagram of Model DU optical system, Beckman manual, 1954 "Light from the tungsten lamp is focused by the condensing mirror and directed in a beam to the diagonal slit entrance mirror.

  5. Spectroelectrochemistry - Wikipedia

    en.wikipedia.org/wiki/Spectroelectrochemistry

    Infrared spectroscopy is based on the fact that molecules absorb electromagnetic radiation at characteristic frequencies related to their vibrational structure. Infrared (IR) spectroelectrochemistry is a technique that allows the characterization of molecules based on the resistance, stiffness and number of bonds present.

  6. Optical spectrometer - Wikipedia

    en.wikipedia.org/wiki/Optical_spectrometer

    In modern spectrographs in the UV, visible, and near-IR spectral ranges, the spectrum is generally given in the form of photon number per unit wavelength (nm or μm), wavenumber (μm −1, cm −1), frequency (THz), or energy (eV), with the units indicated by the abscissa.

  7. UV-Vis absorption spectroelectrochemistry - Wikipedia

    en.wikipedia.org/wiki/UV-Vis_absorption_spectro...

    It is the link between the electrochemistry and the UV-Vis absorption spectroscopy. [3] Devices to conduct the radiation beam: lenses, mirrors and/or optical fibers. The last ones conduct electromagnetic radiation over great distances with hardly any losses.

  8. Ultraviolet–visible spectroscopy of stereoisomers - Wikipedia

    en.wikipedia.org/wiki/Ultraviolet–visible...

    Ultraviolet–visible spectroscopy (UV–vis) can distinguish between enantiomers by showing a distinct Cotton effect for each isomer. UV–vis spectroscopy sees only chromophores , so other molecules must be prepared for analysis by chemical addition of a chromophore such as anthracene .

  9. UV detectors - Wikipedia

    en.wikipedia.org/wiki/UV_detectors

    The variable UV-Vis absorbance detector's optical bench is showing how the flow cell is positioned after the optical system, including the monochromator, which typically has a physical slit and a moving grating, so it is illuminated by a selected wavelength, reaching a photo-diode.