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Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
The first combination of a confocal microscope with specialized computer hardware that enabled digital image processing was presented by Van der Voort et al. (1985). In the late 1980s, Biorad introduced the first commercial laser scanning confocal microscope to the market, followed by Carl Zeiss and Leica soon after. All of these instruments ...
IMOD will also open TIF files and includes a set of programs to convert between image formats including common microscopy formats like ".raw" and ".dm4". Vector Format : IMOD saves and opens vector data in the form of contour (polygons) and meshes in an IMOD binary file format , typically with a ".mod" or ".fid" extension.
Scanning laser ophthalmoscopy developed as a method to view a distinct layer of the living eye at the microscopic level. The use of confocal methods to diminish extra light by focusing detected light through a small pinhole made possible the imaging of individual layers of the retina with greater distinction than ever before. [4]
The development of Aphelion started in 1995 as a joint project of a French company, ADCIS S.A., [2] and an American company, Amerinex Applied Imaging, Inc. (AAI) [3] Aphelion's image processing and analysis functions were made from operators available from the KBVision software developed and sold by Amerinex's predecessor, Amerinex Artificial Intelligence Inc.
DAVID Laserscanner is a software package for low-cost 3D laser scanning. It allows scanning and digitizing of three-dimensional objects using a camera (e.g. a web cam), a hand-held line laser (i.e. one that projects a line, not just a point), and two plain boards in the background. The software generates 3D data in real time and shows them on ...
Laser scanning is the controlled deflection of laser beams, visible or invisible. [1] Scanned laser beams are used in some 3-D printers, in rapid prototyping, in machines for material processing, in laser engraving machines, in ophthalmological laser systems for the treatment of presbyopia, in confocal microscopy, in laser printers, in laser shows, in Laser TV, and in barcode scanners.
Amira (ah-MEER-ah) is a software platform for visualization, processing, and analysis of 3D and 4D data. It is being actively developed by Thermo Fisher Scientific in collaboration with the Zuse Institute Berlin (ZIB), and commercially distributed by Thermo Fisher Scientific — together with its sister software Avizo.