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  2. Transcriptomics technologies - Wikipedia

    en.wikipedia.org/wiki/Transcriptomics_technologies

    The information content of an organism is recorded in the DNA of its genome and expressed through transcription. Here, mRNA serves as a transient intermediary molecule in the information network, whilst non-coding RNAs perform additional diverse functions. A transcriptome captures a snapshot in time of the total transcripts present in a cell ...

  3. Transcriptome - Wikipedia

    en.wikipedia.org/wiki/Transcriptome

    The term transcriptome is a portmanteau of the words transcript and genome; it is associated with the process of transcript production during the biological process of transcription. The early stages of transcriptome annotations began with cDNA libraries published in the 1980s. Subsequently, the advent of high-throughput technology led to ...

  4. RNA-Seq - Wikipedia

    en.wikipedia.org/wiki/RNA-Seq

    [136] [137] Limitations of RNA variant identification include that it only reflects expressed regions (in humans, <5% of the genome), could be subject to biases introduced by data processing (e.g., de novo transcriptome assemblies underestimate heterozygosity [138]), and has lower quality when compared to direct DNA sequencing.

  5. Genomics - Wikipedia

    en.wikipedia.org/wiki/Genomics

    The DNA sequence assembly alone is of little value without additional analysis. [9] Genome annotation is the process of attaching biological information to sequences, and consists of three main steps: [68] identifying portions of the genome that do not code for proteins; identifying elements on the genome, a process called gene prediction, and

  6. Genome evolution - Wikipedia

    en.wikipedia.org/wiki/Genome_evolution

    The principal forces of evolution in prokaryotes and their effects on archaeal and bacterial genomes. The horizontal line shows archaeal and bacterial genome size on a logarithmic scale (in megabase pairs) and the approximate corresponding number of genes (in parentheses).The effects of the main forces of prokaryotic genome evolution are denoted by triangles that are positioned, roughly, over ...

  7. Single-cell sequencing - Wikipedia

    en.wikipedia.org/wiki/Single-cell_sequencing

    Single-cell DNA methylation sequencing has been widely used to explore epigenetic differences in genetically similar cells. To validate these methods during their development, the single-cell methylome data of a mixed population were successfully classified by hierarchal clustering to identify distinct cell types. [ 35 ]

  8. 1000 Plant Genomes Project - Wikipedia

    en.wikipedia.org/wiki/1000_Plant_Genomes_Project

    As of 2002, the number of classified green plant species was estimated to be around 370,000, however, there are probably many thousands more yet unclassified. [10] Despite this number, very few of these species have detailed DNA sequence information to date; 125,426 species in GenBank, as of 11 April 2012, [11] but most (>95%) having DNA sequence for only one or two genes. "...almost none of ...

  9. Conserved sequence - Wikipedia

    en.wikipedia.org/wiki/Conserved_sequence

    In evolutionary biology, conserved sequences are identical or similar sequences in nucleic acids (DNA and RNA) or proteins across species (orthologous sequences), or within a genome (paralogous sequences), or between donor and receptor taxa (xenologous sequences). Conservation indicates that a sequence has been maintained by natural selection.

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