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  2. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    DNA extraction. The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. [1] DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other ...

  3. Boom method - Wikipedia

    en.wikipedia.org/wiki/Boom_method

    The Boom method (Boom nucleic acid extraction method) [1][2][3][4][5][6][7][8] is a solid phase extraction method for isolating nucleic acids (NA) [9] from biological samples. Silica beads are a key element to this method, which are capable of binding the nucleic acids in the presence of a chaotropic substance according to the chaotropic effect ...

  4. Plasmid preparation - Wikipedia

    en.wikipedia.org/wiki/Plasmid_preparation

    Plasmid miniprep. 0.8% agarose gel ethidium bromide -stained. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology. [1][2] Many methods have been developed to purify ...

  5. DNA microarray - Wikipedia

    en.wikipedia.org/wiki/DNA_microarray

    How to use a microarray for genotyping. The video shows the process of extracting genotypes from a human spit sample using microarrays. Genotyping is a major use of DNA microarrays, but with some modifications they can also be used for other purposes such as measurement of gene expression and epigenetic markers.

  6. RNA extraction - Wikipedia

    en.wikipedia.org/wiki/RNA_extraction

    RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA. [1] Several methods are used in molecular biology to isolate RNA from samples, the most common of these is guanidinium thiocyanate-phenol ...

  7. DNA - Wikipedia

    en.wikipedia.org/wiki/DNA

    In biology, parts of the DNA double helix that need to separate easily, such as the TATAAT Pribnow box in some promoters, tend to have a high AT content, making the strands easier to pull apart. [29] In the laboratory, the strength of this interaction can be measured by finding the melting temperature T m necessary to break half of the hydrogen ...

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