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  2. Western blot - Wikipedia

    en.wikipedia.org/wiki/Western_blot

    Western blot workflow. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]

  3. Western blot normalization - Wikipedia

    en.wikipedia.org/wiki/Western_blot_normalization

    Normalization of Western blot data is an analytical step that is performed to compare the relative abundance of a specific protein across the lanes of a blot or gel under diverse experimental treatments, or across tissues or developmental stages.

  4. Blot (biology) - Wikipedia

    en.wikipedia.org/wiki/Blot_(biology)

    A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. [1]

  5. Far-western blot - Wikipedia

    en.wikipedia.org/wiki/Far-Western_blot

    The far-western blot, or far-western blotting, is a molecular biological method based on the technique of western blot to detect protein-protein interaction in vitro. [1] [2] Whereas western blot uses an antibody probe to detect a protein of interest, far-western blot uses a non-antibody probe which can bind the protein of interest. Thus ...

  6. Immunohistochemistry - Wikipedia

    en.wikipedia.org/wiki/Immunohistochemistry

    Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.

  7. Immunoprecipitation - Wikipedia

    en.wikipedia.org/wiki/Immunoprecipitation

    After washing, the precipitated protein(s) are eluted and analyzed by gel electrophoresis, mass spectrometry, western blotting, or any number of other methods for identifying constituents in the complex. Protocol times for immunoprecipitation vary greatly due to a variety of factors, with protocol times increasing with the number of washes ...

  8. Northwestern blot - Wikipedia

    en.wikipedia.org/wiki/Northwestern_blot

    The northwestern blot allows for detection of identified proteins in a way that is inexpensive. The blot is typically a first step in research, as it allows for the identification of the approximate molecular weights, once the molecular weight is known it allows for further research or purification through other methods like chromatography.

  9. Methods (journal) - Wikipedia

    en.wikipedia.org/wiki/Methods_(journal)

    Methods is a peer-reviewed scientific journal covering research on techniques in the experimental biological and medical sciences. It absorbed two journals, ImmunoMethods and NeuroProtocols . Abstracting and indexing