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Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
Besides the culture of well-established immortalised cell lines, cells from primary explants of a plethora of organisms can be cultured for a limited period of time before senescence occurs (see Hayflick's limit). Cultured primary cells have been extensively used in research, as is the case of fish keratocytes in cell migration studies. [88 ...
Immortalised cell lines are widely used as a simple model for more complex biological systems – for example, for the analysis of the biochemistry and cell biology of mammalian (including human) cells. [2] The main advantage of using an immortal cell line for research is its immortality; the cells can be grown indefinitely in culture.
At the start of his experiment he named the primary culture "phase one". Phase two is defined as the period when cells are proliferating; Hayflick called this the time of "luxuriant growth". After months of doubling the cells eventually reach phase three, a phenomenon he named "senescence", where cell replication rate slows before halting ...
These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. [8] Thus, in its broader sense, "tissue culture" is often used interchangeably with "cell culture".
To create an embryonic stem cell line, the inner cell-mass is removed from the blastocyst, separated from the trophoectoderm, and cultured on a layer of supportive cells in vitro. In the derivation of human embryonic stem cell lines, embryos left over from in vitro fertilization (IVF) procedures are used. The fact that the blastocyst is ...
The cell is the fundamental unit of structure and function in all living organisms. [14] The activity of an organism depends on the total activity of independent cells. [15] Energy flow (metabolism and biochemistry) occurs within cells. Cells contain DNA which is found specifically in the chromosome and RNA found in the cell nucleus and ...
The eukaryotic cell seems to have evolved from a symbiotic community of prokaryotic cells. DNA-bearing organelles like mitochondria and chloroplasts are remnants of ancient symbiotic oxygen-breathing bacteria and cyanobacteria, respectively, where at least part of the rest of the cell may have been derived from an ancestral archaean prokaryote ...