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The most probable number method, otherwise known as the method of Poisson zeroes, is a method of getting quantitative data on concentrations of discrete items from positive/negative (incidence) data. Purpose
Suspend the dry ingredients in one liter of cold distilled water. Gently heat until completely dissolved and distribute 9 mL into test tubes with an inverted Durham tube. Sterilize in an autoclave at 121°C for 15 minutes. If needed, prepare multi-strength broth weighing the appropriate quantity of the dry medium. The final pH is 6.9 ± 0.1. [3]
Instead of colony-forming units, the parameters Most Probable Number (MPN) and Modified Fishman Units (MFU) [29] can be used. The Most Probable Number method counts viable cells and is useful when enumerating low concentrations of cells or enumerating microbes in products where particulates make plate counting impractical. [30]
ISO 7899 Water quality – Detection and enumeration of intestinal enterococci ISO 7899-1:1998 Part 1: Miniaturized method (Most Probable Number) for surface and waste water; ISO 7899-2:2000 Part 2: Membrane filtration method; ISO 7919 Mechanical vibration – Evaluation of machine vibration by measurements on rotating shafts
ISO 9308-2:2012 Part 2: Most probable number method; ISO 9308-3:1998 Part 3: Miniaturized method (Most Probable Number) for the detection and enumeration of E. coli in surface and waste water; ISO 9314 Information processing systems Fibre Distributed Data Interface (FDDI) ISO 9314-1:1989 Part 1: Token Ring Physical Layer Protocol (PHY)
One of the oldest methods is called the multiple tube method. [3] In this method a measured sub-sample (perhaps 10 ml) is diluted with 100 ml of sterile growth medium and an aliquot of 10 ml is then decanted into each of ten tubes. The remaining 10 ml is then diluted again and the process repeated.
3.3 Water activity. 3.4 Oxygen. 3. ... (colony counting), or indirect and bulk (most probable number ... Batch culture is the most common laboratory growth method in ...
In the Plate Count Method, the sample of drug product to be tested and Soybean-Casein Digest Broth is poured into a Petri dish. [4] The Petri dish is then incubated. The most probable number method (MPN) can also be performed for products considered to have a low bioburden [clarification needed]. The MPN is considered to be one of the least ...