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The pair of chains have a radius of 10 Å (1.0 nm). [9] According to another study, when measured in a different solution, the DNA chain measured 22–26 Å (2.2–2.6 nm) wide, and one nucleotide unit measured 3.3 Å (0.33 nm) long. [10] The buoyant density of most DNA is 1.7g/cm 3. [11]
The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size.
Deoxyribonucleic acid (DNA) is a nucleic acid containing the genetic instructions used in the development and functioning of all known living organisms. The chemical DNA was discovered in 1869, but its role in genetic inheritance was not demonstrated until 1943. The DNA segments that carry this genetic information are called genes.
Log–log plot of the total number of annotated proteins in genomes submitted to GenBank as a function of genome size. Genome size is the total number of the DNA base pairs in one copy of a haploid genome. Genome size varies widely across species. Invertebrates have small genomes, this is also correlated to a small number of transposable elements.
A-DNA is broader and more compressed along its axis than B-DNA. [2] [3] The identifiable characteristic of A-DNA X-ray crystallography is the hole in the center. [2] A-DNA has a C3'-endo pucker, which refers to the C3' carbon in the furanose ring being below the sugar plane.
A multiple choice question, with days of the week as potential answers. Multiple choice (MC), [1] objective response or MCQ(for multiple choice question) is a form of an objective assessment in which respondents are asked to select only the correct answer from the choices offered as a list.
To date, several methods for gene synthesis have been described, such as the ligation of phosphorylated overlapping oligonucleotides, [2] [3] the Fok I method [4] and a modified form of ligase chain reaction for gene synthesis. Additionally, several PCR assembly approaches have been described. [9]
The main rad3 effector is the kinase Chk1, which is required for the G2-M arrest in response to DNA-damaging agents. [9] Chk1 is an effector protein kinase that maintains mitotic cyclin in an inactive state and is phosphorylated by rad3 between S phase and mitosis, implicating its specific role in G2 arrest. [ 10 ]