Ads
related to: housekeeping genes for western blot- iBright Analysis Software
User-focused analysis software.
Desktop and cloud-based versions.
- Western Blotting Handbook
New, updated technical guide.
Download your copy now.
- iBright Imaging Systems
Find which model is right for you.
iBright CL750, CL1500, or FL1500.
- Examine Features
Discover our suite of features.
Learn about the benefits of iBright
- Explore our Brochure
See what iBright has to offer.
Download, print, and share.
- 21 CFR part 11 Support
Learn about compliance support.
Security, auditing & e-signatures.
- iBright Analysis Software
Search results
Results From The WOW.Com Content Network
Housekeeping genes and proteins, including β-Actin, GAPDH, HPRT1, and RPLP1, are often used as internal controls in western blots because they are thought to be expressed constitutively, at the same levels, across experiments.
Housekeeping genes account for majority of the active genes in the genome, and their expression is obviously vital to survival. The housekeeping gene expression levels are fine-tuned to meet the metabolic requirements in various tissues.
Actin beta is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa. [citation needed]
Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. For this reason, GAPDH is commonly used by biological researchers as a loading control for western blot and as a control for qPCR.
Western blot workflow. The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. [1]
The following represent genes that should probably not be used for reference purposes: GUSB, [5] RPLP0, [5] TFRC, [5] GAPDH, HSP90, and β-actin. Although they were once considered as "housekeeping genes," recent data suggests that they are not as reliable as once thought. [1] [2]
For genes encoding proteins, the expression level can be directly assessed by a number of methods with some clear analogies to the techniques for mRNA quantification. One of the most commonly used methods is to perform a Western blot against the protein of interest. [130] This gives information on the size of the protein in addition to its ...
Due to the sequence conservation in housekeeping genes, MLST sometimes lacks the discriminatory power to differentiate bacterial strains, which limits its use in epidemiological investigations. To improve the discriminatory power of MLST, a multi-virulence-locus sequence typing (MVLST) approach has been developed using Listeria monocytogenes ...
Ad
related to: housekeeping genes for western blot