Search results
Results From The WOW.Com Content Network
The isoelectric point (pI, pH(I), IEP), is the pH at which a molecule carries no net electrical charge or is electrically neutral in the statistical mean. The standard nomenclature to represent the isoelectric point is pH(I). [1] However, pI is also used. [2] For brevity, this article uses pI.
Amino acids have zero mobility in electrophoresis at their isoelectric point, although this behaviour is more usually exploited for peptides and proteins than single amino acids. Zwitterions have minimum solubility at their isoelectric point, and some amino acids (in particular, with nonpolar side chains) can be isolated by precipitation from ...
It works on almost any kind of charged molecule—including small inorganic anions, [2] large proteins, [3] small nucleotides, [4] and amino acids. However, ion chromatography must be done in conditions that are one pH unit away from the isoelectric point of a protein. [5] The two types of ion chromatography are anion-exchange and cation-exchange.
The isoelectric point of albumin is 4.7. [8] Alpha-fetoprotein is a fetal plasma protein that binds various cations, fatty acids and bilirubin. Vitamin D-binding protein binds to vitamin D and its metabolites, as well as to fatty acids. Not much is known about afamin. It seems to carry lipidated Wnt proteins and Vitamin E around. [9]
The isoionic point is the pH value at which a zwitterion molecule has an equal number of positive and negative charges and no adherent ionic species. It was first defined by S.P.L. Sørensen , Kaj Ulrik Linderstrøm-Lang and Ellen Lund in 1926 [ 1 ] and is mainly a term used in protein sciences.
Toggle the table of contents. ... Isoelectric Point [8] 979 59 ... The protein that is encoded by SMCO4 is rich in phosphorylable amino acids and has a high pH. There ...
The isoelectric point of polyampholytes is the pH at which the polymer exhibits no net charge, balancing its positive and negative charges. This point is important because it dictates the net charge of polyampholyte macromolecules at different pH levels. At a pH less than the isoelectric point, the macromolecules carry a positive charge, while ...
The side chains of the standard amino acids have a variety of chemical structures and properties, and it is the combined effect of all amino acids that determines its three-dimensional structure and chemical reactivity. [35] The amino acids in a polypeptide chain are linked by peptide bonds between amino and carboxyl