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The second method of histology processing is called frozen section processing. This is a highly technical scientific method performed by a trained histoscientist. In this method, the tissue is frozen and sliced thinly using a microtome mounted in a below-freezing refrigeration device called the cryostat. The thin frozen sections are mounted on ...
The usual histology slice is cut at 5 to 10 micrometres. The surgical specimen is placed on a metal tissue disc which is then secured in a chuck and frozen rapidly to about –20 to –30 °C. The specimen is placed in a gel-like embedding medium, usually OCT which consists of polyethylene glycol and polyvinyl alcohol ; this compound is known ...
Similar to the frozen section procedure employed in medicine, cryosectioning is a method to rapidly freeze, cut, and mount sections of tissue for histology. The tissue is usually sectioned on a cryostat or freezing microtome. [12] The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues.
Block holders (in histopathology) used to hold the tissue blocks during cutting •Refrigerated microtome -do-; a special type that is used during operations to aid the surgeon in demarcating the diseased (specially neoplasms) tissue. •Rocking microtome-do-; a special type •Base sledge microtome-do-; a special type •Ultra microtome
Bread loafing is a common method of processing surgical specimens for histopathology. The process involves cutting the specimen into 3 or more sections. [ 1 ] The cut sections are mounted by embedding in paraffin or frozen medium.
Gross examination of a kidney (right of image) with a renal oncocytoma (left of image).. Gross processing, "grossing" or "gross pathology" is the process by which pathology specimens undergo examination with the bare eye to obtain diagnostic information, as well as cutting and tissue sampling in order to prepare material for subsequent microscopic examination.
Microscopic view of a histologic specimen of human lung tissue stained with hematoxylin and eosin. Automated tissue image analysis or histopathology image analysis ( HIMA ) is a process by which computer-controlled automatic test equipment is used to evaluate tissue samples, using computations to derive quantitative measurements from an image ...
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]