Ads
related to: does azo cause positive nitrite stain
Search results
Results From The WOW.Com Content Network
When sulfanilamide is added, the nitrite ion reacts with it in the Griess diazotization reaction to form a diazonium salt, which then reacts with N-(1-naphthyl)ethylenediamine in an azo coupling reaction, forming a pink-red azo dye. Using a spectrophotometer, it is possible to quantitatively determine the nitrite concentration. The detection ...
A nitrite test is a standard component of a urinary test strip. A positive test for nitrites in the urine is called nitrituria. This test is commonly used in diagnosing urinary tract infections (UTIs). A positive nitrite test indicates that the cause of the UTI is a Gram-negative organism, most commonly Escherichia coli. The reason for nitrites ...
The presence of nitrites, which causes a pink color on the reagent strip pad, therefore acts as an indicator of urinary tract infection. [51] The nitrite test is quite specific, meaning that someone is likely to have a UTI if it is positive, but it is not sensitive; a negative result does not reliably indicate that the subject does not have a UTI.
Phenazopyridine is an azo dye. [17] [18] Other azo dyes, which were previously used in textiles, printing, and plastic manufacturing, have been implicated as carcinogens that can cause bladder cancer. [19] While phenazopyridine has never been shown to cause cancer in humans, evidence from animal models suggests that it is potentially ...
Many species of nitrifying bacteria have complex internal membrane systems that are the location for key enzymes in nitrification: ammonia monooxygenase (which oxidizes ammonia to hydroxylamine), hydroxylamine oxidoreductase (which oxidizes hydroxylamine to nitric oxide - which is further oxidized to nitrite by a currently unidentified enzyme ...
In organic chemistry, an azo coupling is an reaction between a diazonium compound (R−N≡N +) and another aromatic compound that produces an azo compound (R−N=N−R’).In this electrophilic aromatic substitution reaction, the aryldiazonium cation is the electrophile, and the activated carbon (usually from an arene, which is called coupling agent), serves as a nucleophile.
Azo violet can be synthesised by reacting 4-nitroaniline with nitrous acid (generated in situ with an acid and a nitrite salt) to produce a diazonium intermediate. This is then reacted with resorcinol, dissolved in a sodium hydroxide solution, via an azo coupling reaction. This is consistent with the generalized strategy for preparing azo dyes.
Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the specimens (for positive stains) or background (for negative stains) will be one color. Therefore, simple stains are typically used for viewing only one organism per slide. Differential staining uses multiple stains per ...