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A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. [1]
There are a variety of platforms used to facilitate the growth of three-dimensional cellular structures including scaffold systems such as hydrogel matrices [70] and solid scaffolds, and scaffold-free systems such as low-adhesion plates, nanoparticle facilitated magnetic levitation, [71] hanging drop plates, [72] [73] and rotary cell culture.
3D cell culture methods have been developed to enable research into the behavior of cells in an environment that represents their interactions in-vivo more accurately [5].. 3D cell culturing by magnetic levitation uses biocompatible polymer-based reagents [2] to deliver magnetic nanoparticles to individual cells, so that an applied magnetic driver can levitate cells off the bottom of the cell ...
The group recorded the final cell number as approximately 1.7 x 10 7 cells from a starter batch of only 200,000 cells. When the same group cultured human choriocarcinoma cells on polymeric and silicone polycarbonate capillary membranes totaling less than 3 cm 3 in volume, the cells expanded to an amount approximating 2.17 x 10 8 cells.
[8] [9] Brain-on-a-chip devices can span multiple levels of complexity in terms of cell culture methodology and can include brain parenchyma and/or blood-brain barrier tissues. [10] Devices have been made using platforms that range from traditional 2D cell culture to 3D tissues in the form of organotypic brain slices and more recently organoids.
Micro-mass cultures of C3H-10T1/2 cells at varied oxygen tensions stained with Alcian blue. A commonly applied definition of tissue engineering, as stated by Langer [3] and Vacanti, [4] is "an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve [Biological tissue] function or a ...