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The intensity of the color, and hence the absorption at 540 nm, is directly proportional to the protein concentration, according to the Beer–Lambert law. Despite its name, the reagent does not in fact contain biuret [(H 2 N−CO−) 2 NH]. The test is named so because it also gives a positive reaction to the peptide-like bonds in the biuret ...
Protein dipstick measurements should not be confused with the amount of protein detected on a test for microalbuminuria which denotes values for protein for urine in mg/day versus urine protein dipstick values which denote values for protein in mg/dL. That is, there is a basal level of proteinuria that can occur below 30 mg/day which is ...
Biuret is used as a non-protein nitrogen source in ruminant feed, [5] where it is converted into protein by gut microorganisms. [6] It is less favored than urea, due to its higher cost and lower digestibility [7] but the latter characteristic also slows down its digestion and so decreases the risk of ammonia toxicity.
Reference ranges for urine tests are described below: Measurement Lower limit Upper limit Unit ... Protein: 0: trace amounts [2] Glucose: n/a: 0 / negative [2 ...
Urinalysis, a portmanteau of the words urine and analysis, [1] is a panel of medical tests that includes physical (macroscopic) examination of the urine, chemical evaluation using urine test strips, and microscopic examination.
The method combines the reactions of copper ions with the peptide bonds under alkaline conditions (the Biuret test) with the oxidation of aromatic protein residues. The Lowry method is based on the reaction of Cu +, produced by the oxidation of peptide bonds, with Folin–Ciocalteu reagent (a mixture of phosphotungstic acid and phosphomolybdic acid in the Folin–Ciocalteu reaction).
Urine cytology — cytopathological examination of cells in the urine, used to screen for cancer [21] Urine protein electrophoresis — classification and measurement of different proteins in the urine; used to help diagnose monoclonal gammopathies [22] Urine organic acids, urine amino acids — used to test for some inborn errors of metabolism [8]
Automatic analysis of urine test strips using automated urine test strip analysers is a well-established practice in modern-day urinalysis. They can measure calcium , blood, glucose, bilirubin, urobilinogen, ketones, leukocytes, creatinine , microalbumin , pH, ascorbic acid and protein.