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Due to its short staining time, Diff-Quik stain is often used for initial screening of cytopathology specimens. This staining technique allows the cytotechnologist or pathologist to quickly assess the adequacy of the specimen, identify possible neoplastic or inflammatory changes, and decide whether or not additional staining is required. [4] [9 ...
Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the specimens (for positive stains) or background (for negative stains) will be one color. Therefore, simple stains are typically used for viewing only one organism per slide. Differential staining uses multiple stains per ...
In pathology, the Grocott–Gömöri's methenamine silver stain, abbreviated GMS, is a popular staining method in histology. The stain was originally named after György Gömöri, the Hungarian physician who developed the stain. It is used widely as a screen for fungal organisms. It is particularly useful in staining carbohydrates.
Use of sample-staining methods for use in microbiology, such as simple stains (methylene blue, safranin, crystal violet) and differential stains (negative stains, flagellar stains, endospore stains). Use of a colored (usually blue) or polarizing filter on the light source to highlight features not visible under white light.
Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...
The Wayson stain is a basic fuchsin-methylene blue, ethyl alcohol-phenol microscopic staining procedure. It was originally a modified methylene blue stain used for diagnosing bubonic plague . [ 1 ] With this stain, Yersinia pestis appears purple with a characteristic safety-pin appearance, [ 2 ] which is due to the presence of a central vacuole.
Clusters of bacteria (arrow) shown on Warthin–Starry stain. The Warthin–Starry stain ( WS ) is a silver nitrate -based staining method (a silver stain ) used in histology. It was first introduced in 1920 by American pathologists Aldred Scott Warthin (1866–1931) and Allen Chronister Starry (1890–1973), for the detection of spirochetes .
After five minutes, the slide is removed from the steam, and the paper towel is removed. After cooling, the slide is rinsed with water for thirty seconds. The slide is then stained with diluted safranin for two minutes, which stains most other microorganic bodies red or pink. The slide is then rinsed again, and blotted dry with bibulous paper. [2]