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Electron tomography is an extension of traditional transmission electron microscopy and uses a transmission electron microscope to collect the data. In the process, a beam of electrons is passed through the sample at incremental degrees of rotation around the center of the target sample. This information is collected and used to assemble a ...
As in other electron tomography techniques, the sample is tilted to different angles relative to the electron beam (typically every 2-3 degrees from about −60° to +60°), and an image is acquired at each angle. [5] This tilt-series of images can then be computationally reconstructed into a three-dimensional view of the object of interest. [6]
electron-electron scattering Bhabha scattering: electron-positron scattering Penguin diagram: a quark changes flavor via a W or Z loop Tadpole diagram: One loop diagram with one external leg Self-interaction or oyster diagram An electron emits and reabsorbs a photon Box diagram The box diagram for kaon oscillations: Photon-photon scattering
The structure of the sample may also be changed during the preparation process. Also the field of view is relatively small, raising the possibility that the region analyzed may not be characteristic of the whole sample. There is potential that the sample may be damaged by the electron beam, particularly in the case of biological materials.
Cryogenic electron tomography (cryo-ET), a specialized application where many images are taken of individual samples at various tilt angles, resulting in a 3D reconstruction of a single sample. [27] Electron crystallography, method to determine the arrangement of atoms in solids using a TEM; MicroED, [28] method to determine the structure of ...
Convergent-beam electron diffraction (CBED) is a STEM technique that provides information about crystal structure at a specific point in a sample. In CBED, the width of the area a diffraction pattern is acquired from is equal to the size of the probe used, which can be smaller than 1 Å in an aberration-corrected STEM (see above).
CryoTEM image of GroEL suspended in amorphous ice at 50 000 × magnification Structure of Alcohol oxidase from Pichia pastoris by CryoTEM. Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid ...
Computed tomography (CT) and positron emission tomography (PET) scans are similar to MRI, but rely on different imaging techniques (X-rays and ionizing radiation, respectively). A variation of CT known as contrast CT also requires the subject to take in a contrast medium called a radiocontrast (typically by oral consumption, enema, or injection).