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Critical applications may require monthly service, while research and educational institutions may need only annual service. These are general guidelines and any decision on the appropriate calibration interval should be made carefully and include considerations of the pipette in question (some are more reliable than others), the conditions ...
These pipettes have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a single volume (like a volumetric flask). Typical volumes are 20, 50, and 100 mL. Volumetric pipettes are commonly used to make laboratory solutions from a base stock as well as prepare solutions for titration.
Release the pipette knob slowly to the ready position. Do not remove the tip from the solution. Repeat this process (step 3 and 4) to rinse the tip until the interior wall of the tip is clear. Remove the tip from the solution. Press the pipette knob to the second stop, and completely empty the tip. Release the pipette knob to the ready position.
(These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid pipette (capable of dispensing as little as 10 μL) designed with a circulating liquid tip that generates a self-confining volume in front of its outlet channels. [3]
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop (#3). Withdraw the tip from the liquid. Some liquid will remain inside the tip. The liquid remaining in the tip can be dispensed back into the original solution (#4) or thrown away. Release the pipette thumb to the ready ...
A graduated pipette is a pipette with its volume, in increments, marked along the tube. It is used to accurately measure and transfer a volume of liquid from one container to another. [ 1 ] It is made from plastic or glass tubes and has a tapered tip.
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