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A UV-Vis spectrophotometer is an analytical instrument that measures the amount of ultraviolet (UV) and visible light that is absorbed by a sample. It is a widely used technique in chemistry, biochemistry, and other fields, to identify and quantify compounds in a variety of samples.
Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...
The Cary 14 spectrophotometer was first produced in 1954 by the Applied Physics Corporation, which later was named the Cary Instruments Corporation after co-founder Howard Cary. [1] The instrument was a successor to the Cary 11, which was the first commercially available recording UV/Vis spectrophotometer. [3]
The Spectronic 20 is a brand of single-beam spectrophotometer, designed to operate in the visible spectrum [1] across a wavelength range of 340 nm to 950 nm, with a spectral bandpass of 20 nm. [ 2 ] [ 3 ] It is designed for quantitative absorption measurement at single wavelengths. [ 1 ]
If the instrument is designed to measure the spectrum on an absolute scale rather than a relative one, then it is typically called a spectrophotometer. The majority of spectrophotometers are used in spectral regions near the visible spectrum. A spectrometer that is calibrated for measurement of the incident optical power is called a ...
A photometer is an instrument that measures the strength of electromagnetic radiation in the range from ultraviolet to infrared and including the visible spectrum. Most photometers convert light into an electric current using a photoresistor , photodiode , or photomultiplier .
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