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The modern streak plate method has progressed from the efforts of Robert Koch and other microbiologists to obtain microbiological cultures of bacteria in order to study them. The dilution or isolation by streaking method was first developed in Koch's laboratory by his two assistants Friedrick Loeffler and Georg Theodor August Gaffky.
The streak plate method [2] helps identify the unknown microbe by producing individual colonies on an agar plate which allows for CFU method to be used: Beginning the streak pattern. Label the base of the plate. Then, visualize the plate in four quadrants: top left (I), top right (II), bottom right (III), bottom left (IV).
The phage can then be isolated from the resulting plaques in a lawn of bacteria on a plate. Viral cultures are obtained from their appropriate eukaryotic host cells. The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar ...
An agar plate being viewed in an electronic colony counter Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen ...
Inoculation of a plate culture is done through the streaking technique to make a streak plate. [ 1 ] [ 2 ] [ 4 ] After lifting the lid so that it hovers above the sterile agar plate, the inoculation needle will be streaked across the plate in controlled directions. [ 1 ]
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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.