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The theory of high performance liquid chromatography-HPLC is, at its core, the same as general chromatography theory. [41] This theory has been used as the basis for system-suitability tests, as can be seen in the USP Pharmacopaeia, [ 42 ] which are a set of quantitative criteria, which test the suitability of the HPLC system to the required ...
Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry which utilizes gas-phase ion-molecule reactions at atmospheric pressure (10 5 Pa), [ 1 ][ 2 ] commonly coupled with high-performance liquid chromatography (HPLC). [ 3 ] APCI is a soft ionization method similar to chemical ionization where primary ...
Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high-performance liquid chromatography. In HPLC the sample is forced by a liquid at high pressure (the mobile phase) through a column that is packed with a stationary phase composed of irregularly or ...
The charged aerosol detector (CAD) is a detector used in conjunction with high-performance liquid chromatography (HPLC) and ultra high-performance liquid chromatography (UHPLC) to measure the amount of chemicals in a sample by creating charged aerosol particles which are detected using an electrometer. [1][2][3][4] It is commonly used for the ...
Monolithic HPLC column. A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal structure of the monolithic column is created in such a way that many channels form inside the column. The material inside the column which separates the channels can be porous and functionalized.
Denaturing high performance liquid chromatography. Denaturing High Performance Liquid Chromatography (DHPLC) is a method of chromatography for the detection of base substitutions, small deletions or insertions in the DNA. [1] Due to its speed and high resolution, this method is particularly useful for finding polymorphisms in DNA.
High-pressure liquid chromatography. Add languages. Add links. ... Upload file; Special pages ... Cite this page; Print/export Download as PDF; Printable version ...
A high-performance countercurrent chromatography system. Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquid–liquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force [1] and is used to separate ...