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Focus stacking – also called focal plane merging, z-stacking, [1] or focus blending – is a digital image processing technique which combines multiple images taken at different focus distances to give a resulting image with a greater depth of field (DOF) than any of the individual source images.
Coherent Raman scattering (CRS) microscopy is a multi-photon microscopy technique based on Raman-active vibrational modes of molecules. The two major techniques in CRS microscopy are stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS). SRS and CARS were theoretically predicted and experimentally realized in the 1960s.
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives good optical sectioning.
Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object. Instead, the light wave front information originating from the object is digitally recorded as a hologram , from which a computer calculates the object image by using a numerical reconstruction algorithm .
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye ... (called a Z-stack) plus the ...
The use of two counter-propagating lightsheets helps to reduce typical selective plane illumination microscopy artifacts, like shadowing (see first z-stack above) [8] In addition to counter-propagating lightsheets a setup with detection from two opposing sides has been proposed in 2012.
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