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"Molecular Markers and Genetic Mapping". Oilseeds. Vol. 2 of Genome Mapping and Molecular Breeding in Plants. Springer. p. 88. ISBN 978-3540343875. Li, G.; Quiros, C. F. (2001). "Sequence Related Amplified Polymorphism (SRAP) A New Marker System Based on a Simple PCR Reaction: Its Application to Mapping and Gene Tagging in Brassica".
Molecular breeding is the application of molecular biology tools, often in plant breeding [1] [2] and animal breeding. [3] [4] In the broad sense, molecular breeding can be defined as the use of genetic manipulation performed at the level of DNA to improve traits of interest in plants and animals, and it may also include genetic engineering or gene manipulation, molecular marker-assisted ...
Marker assisted selection or marker aided selection (MAS) is an indirect selection process where a trait of interest is selected based on a marker (morphological, biochemical or DNA/RNA variation) linked to a trait of interest (e.g. productivity, disease resistance, abiotic stress tolerance, and quality), rather than on the trait itself.
For example, DNA is a molecular marker that gives information about the organism from which it was taken. For another example, some proteins can be molecular markers of Alzheimer's disease in a person from which they are taken. [1] Molecular markers may be non-biological. Non-biological markers are often used in environmental studies. [2]
The types of molecular markers used to monitor populations are most commonly mitochondrial, microsatellites or single-nucleotide polymorphisms (SNPs), while earlier studies also used allozyme data. Species gene diversity is also recognized as an important biodiversity metric for implementation of the Convention on Biological Diversity. [1]
In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. Such genes are called reporters because the characteristics they confer on organisms expressing them are easily identified and measured, or because ...
Molecular diagnostics is a collection of techniques used to analyze biological markers in the genome and proteome, and how their cells express their genes as proteins, applying molecular biology to medical testing.
The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology [1] and microbiology. [2] Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique.