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Leukocyte esterase (LE) is an esterase (a type of enzyme) produced by leukocytes (white blood cells). A leukocyte esterase test (LE test) is a urine test for the presence of white blood cells and other abnormalities associated with infection. White blood cells in the urine can indicate a urinary tract infection (UTI). Positive test results may ...
Leukocyte esterase, an enzyme found in granulocytes, is measured to estimate the concentration of white blood cells. [53] The action of the enzyme on chemicals in the test pad ends in the creation of a purple azo dye . [ 54 ]
The urine test strip reaction is based on the action of leukocyte esterase in catalysing the hydrolysis of an ester of indolecarboxylic acid. The indoxyl that is liberated combines with a diazonium salt in order to produce a violet coloured azole dye. [21] 1) Reaction catalysed by leukocyte esterase
In the elderly, the leukocyte esterase test is often positive even in the absence of an infection. [citation needed] The urine dipstick test is readily available and provides fast, but often unreliable results. Some organisms such as chlamydia and Ureaplasma urealyticum will produce a negative leukocyte esterase reaction.
In complicated or questionable cases, it may be useful to confirm the diagnosis via urinalysis, looking for the presence of urinary nitrites, white blood cells (leukocytes), or leukocyte esterase. [54] Another test, urine microscopy, looks for the presence of red blood cells, white blood cells, or bacteria.
Reference ranges for urine tests are described below: Measurement Lower limit Upper limit Unit ... Leukocytes: n/a: 0 / negative [2] See also. Reference range;
It has been arbitrarily assumed that a number of over 4-5 leukocytes in the field of vision of the microscope indicates leukocyturia. [ 4 ] At the moment, there are also quick test strips available, allowing after wetting a special diagnostic bar, the detection of granulocytes in the urine, as evidenced by the color change of the test strip. [ 5 ]
Contemporary urine testing uses a range of methods to investigate the physical and biochemical properties of the urine. For instance, the results of the routine urinalysis can provide information about the functioning of the kidneys and urinary system ; suggest the presence of a urinary tract infection (UTI); and screen for possible diabetes or ...