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  2. Nuclease S1 - Wikipedia

    en.wikipedia.org/wiki/Nuclease_S1

    Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses the following chemical reaction Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products

  3. Upstream and downstream (DNA) - Wikipedia

    en.wikipedia.org/wiki/Upstream_and_downstream_(DNA)

    Each strand of DNA or RNA has a 5' end and a 3' end, so named for the carbon position on the deoxyribose (or ribose) ring. By convention, upstream and downstream relate to the 5' to 3' direction respectively in which RNA transcription takes place. [1] Upstream is toward the 5' end of the RNA molecule, and downstream is toward the 3

  4. S1 domain - Wikipedia

    en.wikipedia.org/wiki/S1_domain

    The S1 domain is a protein domain that was originally identified in ribosomal protein S1 but is found in a large number of RNA-associated proteins. The structure of the S1 RNA-binding domain from the Escherichia coli polynucleotide phosphorylase has been determined using NMR methods and consists of a five-stranded antiparallel beta barrel.

  5. Biochemical engineering - Wikipedia

    en.wikipedia.org/wiki/Biochemical_engineering

    Bioreactor. Biochemical engineering, also known as bioprocess engineering, is a field of study with roots stemming from chemical engineering and biological engineering.It mainly deals with the design, construction, and advancement of unit processes that involve biological organisms (such as fermentation) or organic molecules (often enzymes) and has various applications in areas of interest ...

  6. Untranslated region - Wikipedia

    en.wikipedia.org/wiki/Untranslated_region

    Although they are called untranslated regions, and do not form the protein-coding region of the gene, uORFs located within the 5' UTR can be translated into peptides. [1] The 5' UTR is upstream from the coding sequence. Within the 5' UTR is a sequence that is recognized by the ribosome which allows the ribosome to bind and initiate translation.

  7. Bioprocess - Wikipedia

    en.wikipedia.org/wiki/Bioprocess

    The upstream part of a bioprocess refers to the first step in which microbes/cells are grown, e.g. bacterial or mammalian cell lines (see cell culture), in bioreactors. Upstream processing involves all the steps related to inoculum development, media development, improvement of inoculum by genetic engineering process, optimization of growth ...

  8. Upstream and downstream - Wikipedia

    en.wikipedia.org/wiki/Upstream_and_downstream

    Upstream and Downstream, the notional directions which lie "towards" and "away from" the author of a piece of software, relative to the speaker See also [ edit ]

  9. Prokaryotic small ribosomal subunit - Wikipedia

    en.wikipedia.org/wiki/Prokaryotic_small...

    The 30S subunit is an integral part of mRNA translation.It binds three prokaryotic initiation factors: IF-1, IF-2, and IF-3. [3]A portion of the 30S subunit (the 16S rRNA) guides the initiating start codon (5′)-AUG-(3′) of mRNA into position by recognizing the Shine-Dalgarno sequence, a complementary binding site about 8 base pairs upstream from the start codon. [4]

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