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"Yakety Sax" is often used in television and film as a soundtrack for outlandishly humorous situations. It was frequently used to accompany comedic sketches, particularly the time-lapse , rapidly-paced silent chase skit that came at the end of almost every episode of The Benny Hill Show . [ 17 ]
Other transcription factors differentially regulate the expression of various genes by binding to enhancer regions of DNA adjacent to regulated genes. These transcription factors are critical to making sure that genes are expressed in the right cell at the right time and in the right amount, depending on the changing requirements of the organism.
T-box transcription factor T, also known as Brachyury protein, is encoded for in humans and other apes by the TBXT gene. [5] [6] [7] Brachyury functions as a transcription factor within the T-box family of genes. [8] Brachyury homologs have been found in all bilaterian animals that have been screened, as well as the freshwater cnidarian Hydra. [8]
[53] [54] TBP associates with a host of factors, including the general transcription factors TFIIA, -B, -D, -E, and -H, to form huge multi-subunit pre-initiation complexes on the core promoter. Through its association with different transcription factors, TBP can initiate transcription from different RNA polymerases .
The TRANSFAC database can be used as an encyclopedia of eukaryotic transcription factors. The target sequences and the regulated genes can be listed for each TF, which can be used as benchmark for TFBS recognition tools or as training sets for new transcription factor binding sites (TFBS) recognition algorithms. [ 12 ]
A transcription factor is a protein that binds to specific DNA sequences (enhancer or promoter), either alone or with other proteins in a complex, to control the rate of transcription of genetic information from DNA to messenger RNA by promoting (serving as an activator) or blocking (serving as a repressor) the recruitment of RNA polymerase.
That said, to Americans anyway, this music is almost synonymous with Benny Hill. It's a bit like Sousa's "Liberty Bell," which few people could identify by name but, once heard, is immediately recognized as the "Monty Python" TV theme. To many people, "Yakety Sax" is "that crazy music they play at the end of every Benny Hill show."
The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the DNA-binding domain (DBD or often also abbreviated as BD) and activating domain (AD).