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Phenolphthalein is used in toys, for example as a component of disappearing inks, or disappearing dye on the "Hollywood Hair" Barbie hair. In the ink, it is mixed with sodium hydroxide, which reacts with carbon dioxide in the air. This reaction leads to the pH falling below the color change threshold as hydrogen ions are released by the reaction:
In order to generate the intense pink color indicative of a positive test, the reduced phenolphthalein must be oxidized back to its normal, colored form. In the relevant reaction, hydrogen peroxide reacts with the hemoglobin in the blood. Phenolphthalein does not directly participate in this process; instead, it acts as an external source of ...
Chemical structure of phenolphthalein, a common phthalein dye. Phthalein dyes are a class of dyes mainly used as pH indicators, due to their ability to change colors depending on pH. [1] They are formed by the reaction of phthalic anhydride with various phenols. They are a subclass of triarylmethane dyes. Common phthalein dyes include ...
For optimal accuracy, the color difference between the two species should be as clear as possible, and the narrower the pH range of the color change the better. In some indicators, such as phenolphthalein, one of the species is colorless, whereas in other indicators, such as methyl red, both species confer a color. While pH indicators work ...
This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). Since the color of phenol red can interfere with some spectrophotometric and fluorescent assays, many types of tissue culture media are also available ...
Leaves change color in the fall because their chromophores (chlorophyll molecules) break down and stop absorbing red and blue light. [1] A chromophore is a molecule which absorbs light at a particular wavelength and reflects color as a result. Chromophores are commonly referred to as colored molecules for this reason.
Its sharp and easily detectable colour changes makes phenolphthalein a valuable tool for determining the endpoint of acid-base titrations, as a precise pH change signifies the completion of the reaction. When a weak acid reacts with a weak base, the equivalence point solution will be basic if the base is stronger and acidic if the acid is stronger.
An intermediate of the deprotonation mechanism is responsible for the greenish color in neutral solution. [ 2 ] The protonated form of bromothymol blue has its peak absorption at 427 nm thus transmitting yellow light in acidic solutions, while the deprotonated form has its peak absorption at 602 nm thus transmitting blue light in more basic ...