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The Kastle–Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase -like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into ...
Phenolphthalein (/ f ɛ ˈ n ɒ l (f) θ ə l iː n / [citation needed] feh-NOL(F)-thə-leen) is a chemical compound with the formula C 20 H 14 O 4 and is often written as "HIn", "HPh", "phph" or simply "Ph" in shorthand notation. Phenolphthalein is often used as an indicator in acid–base titrations.
Chemical structure of phenolphthalein, a common phthalein dye. Phthalein dyes are a class of dyes mainly used as pH indicators, due to their ability to change colors depending on pH. [1] They are formed by the reaction of phthalic anhydride with various phenols. They are a subclass of triarylmethane dyes. Common phthalein dyes include ...
o-Cresolphthalein is a phthalein dye used as a pH indicator in titrations. It is insoluble in water but soluble in ethanol. Its solution is colourless below pH 8.2, and purple above 9.8. Its molecular formula is C 22 H 18 O 4. It is used medically to determine calcium levels in the human body, or to synthesize polyamides or polyimides.
Three different points in an acid-base titration using phenolphthalein as the indicator. Phenolphthalein is widely recognized as one of the most commonly used acid-base indicators in chemistry. [12] Its popularity is because of its effectiveness in a broad pH range and its distinct colour transitions. [12]
A roll of universal indicator paper Colors of universal indicator. A universal indicator is a pH indicator made of a solution of several compounds that exhibit various smooth colour changes over a wide range pH values to indicate the acidity or alkalinity of solutions. A universal indicator can be in paper form or present in a form of a ...
Reactions with indicators: bases turn red litmus paper blue, phenolphthalein pink, keep bromothymol blue in its natural colour of blue, and turn methyl orange-yellow. The pH of a basic solution at standard conditions is greater than seven. Bases are bitter. [5]
It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations. In clinical practise, it is commonly used as a diagnostic technique. The most common use of bromocresol green is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease.